CABRI:ICLC |
Accession_number |
ICLC HTL14005
|
Cell_line_name |
AC
|
Brief_description |
Species: human; Tissue: blood; Transformed by: Epstein-Barr
|
Description |
Species: human female; Tissue: blood; Transformed by: Epstein-Barr
|
Depositor |
Dr. M. C. Mingari, IRCCS AOU San Martino - IST, Genoa, Italy
|
Bibliographic_reference |
-
|
Morphology |
lymphoblast like, grown in suspension
|
Culture_conditions |
continuous culture; RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split 1:2-3. 37C, 5% CO2
|
Viruses |
Search for viruses was not performed
|
Release_conditions |
Cell line available for distribution. For non-commercial investigative use only
|
Hazard |
Handle under Biosafety Level 2 containment
|
Freezing_medium |
95% FCS + 5% DMSO
|
Sterility |
mycoplasma negative, HOECHST and PCR
|
Validation_assays |
HLA typing: A2, B8, B35, Cw2, Cw7
|
Comments |
Originator of cell line: Dr. Gabriella Pietra.
|