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Entry from: ICLC
 
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CABRI:ICLC
Accession_number ICLC HTL14005
Cell_line_name AC
Brief_description Species: human; Tissue: blood; Transformed by: Epstein-Barr
Description Species: human female; Tissue: blood; Transformed by: Epstein-Barr
Depositor Dr. M. C. Mingari, IRCCS AOU San Martino - IST, Genoa, Italy
Bibliographic_reference -
Morphology lymphoblast like, grown in suspension
Culture_conditions continuous culture; RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split 1:2-3. 37C, 5% CO2
Viruses Search for viruses was not performed
Release_conditions Cell line available for distribution. For non-commercial investigative use only
Hazard Handle under Biosafety Level 2 containment
Freezing_medium 95% FCS + 5% DMSO
Sterility mycoplasma negative, HOECHST and PCR
Validation_assays HLA typing: A2, B8, B35, Cw2, Cw7
Comments Originator of cell line: Dr. Gabriella Pietra.
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